Taxol Biosynthesis
نویسندگان
چکیده
منابع مشابه
Taxol biosynthesis: an update.
The novel diterpenoid taxol (paclitaxel) is now well-established as a potent chemotherapeutic agent. Total synthesis of the drug is not commercially feasible and, in the foreseeable future, the supply of taxol and its synthetically useful progenitors must rely on biological methods of production. The first three steps of taxol biosynthesis have been defined and the responsible enzymes described...
متن کاملCloning and sequencing of hydroxylase genes involved in taxol biosynthesis.
Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxus chinensis mainly by the single specific-primer PCR (SSP-PCR) method. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol biosynthesis. These findings imply that the two new genes are clos...
متن کاملTaxol biosynthesis: tyrocidine synthetase A catalyzes the production of phenylisoserinyl CoA and other amino phenylpropanoyl thioesters.
In Taxus plants the biosynthesis of the pharmaceutical paclitaxel includes the transfer of β-amino phenylpropanoyls from coenzyme A to the diterpenoid baccatin III by an acyl CoA-dependent acyltransferase. Several enzymes on the pathway are known, yet a few remain unidentified, including the putative ligase that biosynthesizes key β-amino phenylpropanoyl CoAs. The multienzyme, nonribosomal pept...
متن کاملTaxol biosynthesis: Taxane 13 -hydroxylase is a cytochrome P450-dependent monooxygenase
A central feature in the biosynthesis of Taxol is oxygenation at multiple positions of the taxane core structure, reactions that are considered to be mediated by cytochrome P450-dependent monooxygenases. A PCR-based differential display-cloning approach, using Taxus (yew) cells induced for Taxol production, yielded a family of related cytochrome P450 genes, one of which was assigned as a taxane...
متن کاملComparative transcriptome analysis of non-model Taxus species for taxol biosynthesis
Taxol is a known drug, approved by the food and drug administration in 1992 for the treatment of a wide range of cancers. Taxol is obtained from the taxus plant. Taxus plant has a very long life cycle and the production of taxol from the plant is a tedious process. In this study, we have tried to find new species of taxus which can be able to produce taxol and can overcome the burden of Taxus b...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2004
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2004.02.025